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BSA‑seq and genetic mapping reveals AhRt2 as a candidate gene responsible for red testa of peanut.

Institute of Crop Germplasm Resources (Institute of Biotechnology), Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Shandong Academy of Agricultural Sciences, Jinan, 250100, People's Republic of China. College of Agricultural Science and Technology, Shandong Agriculture and Engineering University, Jinan, 250100, People's Republic of China. College of Tropical Crops, Hainan University, Haikou, 572208, China. Shandong Peanut Research Institute, Qingdao, 266199, Shandong, People's Republic of China. College of Life Sciences, Shandong Normal University, Jinan, 250014, People's Republic of China. Cash Crop Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530007, China. Dr. Rajendra Prasad Central Agricultural University, Pusa, Samsthipur, Bihar, 848125, India. Institute of Crop Germplasm Resources (Institute of Biotechnology), Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Shandong Academy of Agricultural Sciences, Jinan, 250100, People's Republic of China. xingjunw@hotmail.com. College of Life Sciences, Shandong Normal University, Jinan, 250014, People's Republic of China. xingjunw@hotmail.com. Institute of Crop Germplasm Resources (Institute of Biotechnology), Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Shandong Academy of Agricultural Sciences, Jinan, 250100, People's Republic of China. chuanzhiz@126.com. College of Life Sciences, Shandong Normal University, Jinan, 250014, People's Republic of China. chuanzhiz@126.com.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik. 2022;(5):1529-1540
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Abstract

The candidate recessive gene AhRt2 responsible for red testa of peanut was identified through combined BSA-seq and linkage mapping approaches. The testa color of peanuts (Arachis hypogaea L.) is an important trait, and those with red testa are particularly popular owing to the high-anthocyanin content. However, the identification of genes underlying the regulation of the red testa trait in peanut are rarely reported. In order to fine map red testa gene, two F2:4 populations were constructed through the cross of YZ9102 (pink testa) with ZH12 (red testa) and ZH2 (red testa). Genetic analysis indicated that red testa was controlled by a single recessive gene named as AhRt2 (Red testa gene 2). Using BSA-seq approach, AhRt2 was preliminary identified on chromosome 12, which was further mapped to a 530-kb interval using 220 recombinant lines through linkage mapping. Furthermore, functional annotation, expression profiling, and the analyses of sequence variation confirmed that the anthocyanin reductase namely (Arahy.IK60LM) was the most likely candidate gene for AhRt2. It was found that a SNP in the third exon of AhRt2 altered the encoding amino acids, and was associated with red testa in peanut. In addition, a closely linked molecular marker linked with red testa trait in peanut was also developed for future studies. Our results provide valuable insight into the molecular mechanism underlying peanut testa color and present significant diagnostic marker resources for marker-assisted selected breeding in peanut.

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Publication Type : Review

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MeSH terms : Plant Proteins